Leukokinetic Studies. I. A Method for Labeling Leukocytes with Diisopropylfluorophosphate (DFP32)

Leukokinetic studies. I. A method for labeling leukocytes with diisopropyl-fluorophosphate (DFP32).

Leukokinetic Studies. Ii. a Method for Labeling Granulocytes in Vitro with Radioactive Diisopropylfluorophosphate (dfp).

In a previous publication from this laboratory (1), radioactive diisopropylfluorophosphate (DFP32), a potent and irreversible esterase inhibitor, was shown to be a suitable label for granulocytes. Evidence was presented that the label is firmly attached to granulocytes; it does not modify the viability of the cells, and the label is not reutilized when the cells are degraded. Experiments design...

A method for leukokinetic study in the nonsteady state.

In these studies, the DF32P leukokinetic is obtained. This technique has been technique has been modified so that successfully employed in the study of the neutrophil kinetics may be studied in the early phase of cortisol-induced granulononsteady state. In order to achieve this cytosis, allowing measurement of cell modification, an acid citrate solution was flow rate in and out of the circulati...

An Improved Method for Performing Neutrophil

I N 1959, ATHENS AND MAUER and their respective collaborators’2 described methods for labeling polymorphonuclear leukocytes with P 52 tagged diisopropylfluorophosphate ( DFP32 ) and subsequently isolating those cells from whole blood. Using that method, they have contributed most of what is known about the intravascular survival and distribution of neutrophils in normal persons. However, that m...

Leukokinetic Studies. Ix. Experimental Evaluation of a Model of Granulopoiesis.

Kinetic models of granulopoiesis have been constructed from data obtained by labeling neutrophilic granulocytes with tritiated thymidine (1, 2), with radioactive phosphorus (3), and with diisopropyl fluorophosphate (DFP32) (4). In many respects these models are remarkably similar in their portrayal of the concatenated compartments of normal granulopoiesis. However, certain questions, particular...